Effects of Different Dietary Protein Levels on the Secretion and mRNA Expression of IGF-1 and GH in Sheep
YAN Yun-feng1,2，YANG Hua1*，YANG Yong-lin1，PAN Xiao-liang2， ZOU Yun-long3
（1.The Breed & Biotechnology Key Laboratory of Sheep in Bingtuan，Xinjiang Academy of Agricultural and Reclamation Sciences，Shihezi 832000，China；2.College of Animal Science and Technology，Shihezi University，Shihezi 832001，China；3.Life Science and Engineering College of Northwest University for Nationalities，Lanzhou 730000，China）
YAN Yun-feng，YANG Hua，YANG Yong-lin，PAN Xiao-liang， ZOU Yun-long. Effects of Different Dietary Protein Levels on the Secretion and mRNA Expression of IGF-1 and GH in Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(1): 85-95.
［1］MULLER E E，LOCATELLI V，COCCHI D.Neuroendocrine control of growth hormone secretion ［J］.Physiol Rev，1999，79(2)：511-608.
［2］LOUVEAU I，GONDRET F.Regulation of development and metabolism of adipose tissue by growth hormone and the insulin-like growth factor system ［J］.Domest Anim Endocrinol, 2004，27(3)：241-255.
［3］周锦玉.吉林白鹅 IGF-1基因的SNP与产绒性状的相关性研究［D］.长春：吉林农业大学，2007.
ZHOU J Y.Studieson single nucleotide polymorphisms of IGF-1 and the association with down traitsin Jilin White Goose［D］.Changchun：Jilin Agricultural University，2007.(in Chinese)
［4］邢海权，闫梦菲.胰岛素样生长因子IGF-1的研究进展 ［J］.兽医研究，2011，（5）：36-37.
XING H Q，YAN M F.The research progress of insulin-like growth factor-1［J］.Veterinary Research，2011，（5）：36-37.(in Chinese)
［5］单秋枝，仲崇刚.生长激素基因研究进展 ［J］.中国畜禽种业，2008，4（4）：64-66.
SHAN Q Z，ZHONG C G. The research progress of growth hormone gene［J］.The Chinese Livestock and Poultry Breeding，2008，4（4）：64-66.(in Chinese)
［6］闫云峰，杨华，杨永林，等.萨福克羊GH和IGF-1基因组织表达水平的分析 ［J］.家畜生态学报，2014，35（4）：18-22.
YAN YF，YANG H，YANG Y L，et al.Tissue expression analysis of GH and IGF-1 genes in sheep［J］.Acta Ecologiae Animalis Domastici，2014，35（4）：18-22.(in Chinese)
［7］贾斌，席继峰，张苏云，等.绵羊皮肤中GHR、IGF-1和IGF-1R基因表达的发育性变化及品种特点 ［J］.遗传，2006，9：1078-1082.
JIA B，XI J F，ZHANG S Y，et al.The developmental patterns of GH-R，IGF-1 and IGF-IR gene expression in sheep skin［J］.Hereditas(Beijing)，2006，9：1078-1082.(in Chinese)
［8］HARRIS P M，MCBRIDE B W，GURNSEY M P，et al.Direct infusion of a variant of insulin-like growth factor-1 into skin of sheep and effects on local blood flow，amino acid utilisation and cell replication［J］. Endocrinology，1993，139：463-472.
［9］杨华，刘守仁，钟发刚，等.BMPR-IB基因在绵羊不同组织的表达差异性研究 ［J］.中国畜牧杂志，2009，45（11）：6-8.
YANG H，LIU S R，ZHONG F G，et al.Study on expression differencesof BMPR-IB gene in different tissues of sheep［J］.Chinese Journal of Animal Science，2009，45（11）：6-8.(in Chinese)
［10］UVAK K J，SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2 ［-Delta Delta C(T)］ Method ［J］.Methods，2001，25：402-408.
［11］BRAMELD J M，ATKINSON J L，SAUNDERS J C，et al.Effects of growth hormone administration and dietary protein intake on insulin-like growth factor 1 and growth hormone receptor mRNA expression in porcine liver，skeletal muscle，and adipose tissue ［J］.J Anim Sci, 1996，74(8)：1832-1841.
［12］PELL J M，SAUNDERS J C，GILMOUR R S.Differential regulation of transcription initiation from insulin-like growth factor-I(IGF-I) leader exons and of tissue IGF-I expression in response to changed growth hormone and nutritional status in sheep ［J］. Endocrinology，1993，132：1797-1807.
［13］BRUHAT A，JOUSSE C，FAFOURONOUX P.Amino acid limitation regulates gene expression ［J］.Proc Nutr Soc，1999，58：625-632.
［14］CASAS-CARRILLO E，KIRKPATRICK B W，PRILL-ADAMS A，et al.Relationship of growth hormone and insulin-like growth factor-1 genotypes with growth and carcass traits in swine ［J］.Anim Genet, 1997，28(2)：88-93.
［15］BARTKE A.Role of growth hormone and prolactin in the control of reproduction：What are we learning from transgenic and knock-out animals ［J］.Steroids, 1999，64(2)：598-604.
［16］李光玉，高秀华，王凯英.梅花鹿、马鹿营养与其血清 IGF-1 浓度的关系研究 ［J］.特产研究，2004，（1）：1-5.
LI G Y，GAO X H，WANG K Y. Research on the relationship of the serum concentrationsof insulin growth factor-1 and the levels of nutrientsin the Sika Deer and Chinese Wapiti［J］.Special Wild Economic Animal and Plant Research，2004，（1）：1-5.(in Chinese)
［17］张勇，宿永波，朱宇旌.日粮蛋白质水平对猪背最长肌calpain1含量、嫩度及血液激素水平的影响 ［J］.中国饲料，2008，（14）：22-26.
ZHANG Y，XU Y B，ZHU Y J. Effects of diet protein level on calpain1 concentration，meat tendernessin longissimusdorsiand blood hormones［J］.China Feed，2008，（14）：22-26.(in Chinese)
［18］BREIER B H.Regulation of protein and energy metabolism by the somatotropic axis ［J］.Domest Anim Endocrinol，1999，17(2)：209-218.
［19］MAO J N，BURNSIDE J，POSTEL-VINAY M C，et al.Ontogeny of growth hormone receptor gene expression in tissue of growth-selected strains of broiler chickens ［J］.J Endocrinol，1998，156(2)：67-75.
［20］GODOWSKI P J，LEUNG D W，MEACHAM L R，et al.Characterization of the human growth hormone receptor gene and demonstration of a partial gene deletion in two patients with Laron-type dwarfism ［J］.Proc Natl Acad Sci，1989，86(20):8083-8087.
［21］赵茹茜，李四桂.禽类生长轴的发育及其对生长的调节 ［J］.畜牧与兽医，1999，31（4）：35-37.
ZHAO R X，LI S G. The role of poultrygrowth axis in development and regulate growth［J］.Animal Husbandry and Veterinary Medicine，1999，31（4）：35-37.(in Chinese)
［22］PICHA M E，TURANO M J，BECKMAN B R，et al.Endocrine biomarkers of growth and applica- tions to aquaculture：a minireview of growth hormone,insulin-like growth factor(IGF)-1，and IGF-binding proteins as potential growth indicators in fish ［J］.North Am J Aquaculture，2008，70(2)：196-211.
［23］赵咏梅.GH-IGF-1轴的作用机制［J］.四川医学，2005，6（7）：808-810.
ZHAO Y M. The mechanism of GH-IGF-1 axis［J］.Sichuan Medical，2005，6（7）：808-810.(in Chinese)
［24］BRAMELD J M，GILMOUR R S，BUTTERY P J.Glucose and amino acids interact with hormones to control expression of insulin-like growth factor-I and growth hormone receptor mRNA in cultured pig hepatocytes ［J］. J Nutr，1999，129(7)：1298-1306.
［25］STRAUS D S，TAKEMOTO C D.Effect of fasting on insulin-like growth factor-1(IGF-1) and growth hormone receptor mRNA levels and IGF-1 gene transcription in rat liver ［J］.Mol Endocrinol，1990，4(1)：91-100.
［26］VANDEHAAR M J，MOATS-STAATS B M，DAVENPORT M L，et al.Reduced serum concentrations of insulin-like growth factor-1(IGF-1) in protein-restricted growing rats are accompanied by reduced IGF-I mRNA levels in liver and skeletal muscle［J］.J Endocrinol，1991，130(2)：305-312.
［27］PELL J M，BATES P C.Collagen and non-collagen protein turnover in skeletal muscle of growth hormone-treated lambs ［J］.J Endocrinol，1987，115(1)：R1.
［28］刘景云，张英杰，刘月琴.日粮不同蛋白水平对绵羊脂肪和肌肉中 IGF-1基因表达的影响 ［J］.畜牧兽医学报，2009，40（2）：197-202.
LIU J Y，ZHANG Y J ，LIU Y Q.Effects of dif ferent dietary crude protein level on the expression of igf-Ⅰmrna in adipose and muscle tissue of sheep［J］.Acta Veterinaria et Zootechnica Sinica，2009，40（2）：197-202.（in Chinese）
［29］张玲，董飚，段修军，等.日粮粗蛋白质水平对鹅组织 IGF-I mRNA 表达的影响 ［J］.江苏农业学报，2012，27（6）：1330-1334.
ZHANG L， DONG B， DUAN X J，et al.Effects of dietary crude protein levels on IGF-I mRNA expression in some tissues of geese［J］. Jiangsu Journal of Agricultural Science，2012，27（6）：1330-1334.(in Chinese)
［30］赵茹茜，GROSSMANN R，陈杰.营养对肉鸡和蛋鸡生长轴基因表达的影响 ［C］.第四届中国畜牧兽医青年科技工作者学术研讨会，2001：4.
ZHAO R X，GROSSMANN R，CHEN J. The impact of nutrition on gene expression in growth axis of broilers and laying hens［C］.The Proceeding of the Fourth Symposium on Chinese Animal Husbandry and Veterinary Young Scientists，2001：4.(in Chinese)
［31］周其伟，白春生，贾斌，等.中国美利奴绵羊肉用品系生长轴相关基因表达对其十二指肠发育的影响 ［J］.畜牧兽医学报，2010，41（8）：967-973.
ZHOU Q W ，BAI C S，JIA B,et al.Ef fects of growth-related genes expression on duodenal development in chinese merino sheep(meat line)［J］.Acta Veterinaria et Zootechnica Sinica，2010，41（8）：967-973.(in Chinese)
［32］ZHAO R Q，DECUYPERE E，GROSSMANN R.Secretory patterns of plasma GH and IGF-1 conc- entrations in meat-type and laying-type chickens during early post hatch development ［J］.Avian Poult Biol Rev, 1995，(6)：270.
